minuta to insect infestation can be achieved not only by an elevated expression of defense-related genes but also by enhanced metabolic activities.Ībstract = "A subtracted library was constructed from planthopper-infested wild rice (Oryza minuta) by suppression subtractive hybridization in combination with mirror orientation selection. The presence of these expressed sequence tags implies that resistance of O. Functional categorization placed the identified transcripts in the categories of subcellular localization, metabolism, and protein fate. sativa, it also revealed that the expression profiles had a different pattern. Although Southern blot analysis showed similar copy numbers of the screened genes in O. The elevated expression levels and overall profiles over time were verified by Northern blot analysis. On the basis of the signal intensities and expression ratios obtained from experiments performed in triplicate, we selected 383 clones. To screen the differentially expressed transcripts in the library, we applied a cDNA microarray containing 960 random clones in a reverse Northern blot analysis using cDNA probes prepared from the mRNAs of control and planthopper-infested samples. A subtracted library was constructed from planthopper-infested wild rice (Oryza minuta) by suppression subtractive hybridization in combination with mirror orientation selection.
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